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The human CBS and L2HGDH gene cDNAs were synthesized with Twist Bioscience (South San Francisco, CA), with the sgRNA targeting regions replaced with “wobble” mutations

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For instance, vitamin C reduces ferric iron to the more absorbable ferrous form and forms soluble complexes that enhance absorption [277], while vitamin D promotes intestinal calcium absorption by upregulating calcium‐binding protein expression via gene regulation [278]