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In 2017, Neelakantan and colleagues first systematically characterized 5‑amino‑1mq during screening for selective NNMT inhibitors, with findings published in Biochemical Pharmacology - marking the official discovery and public reporting of the compound.Through structural modification of quinoline‑based precursors, the team identified 5‑amino‑1mq as a small, membrane‑permeable molecule with the chemical formula C₁₀H₁₁N₂⁺.With an extremely low IC₅₀ value, it became one of the most promising selective NNMT inhibitors available at the time, with high specificity and no significant inhibition of other metabolically relevant enzymes