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In fact, in the compromised scenario of cholestasis, an inflammatory process is established, with a concomitant increase in levels of acute-phase proteins (APP) like serum TNF-α and biliary phospholipase A2, resulting in a further increase in oxidative stress highlighted by raised levels of MDA in the bile [176]

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Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions