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Rather than elute the protein with glutathione buffer, a PBS solution containing the enzyme is loaded onto the column and incubated for several hours at room temperature (4 °C for PreScission protease)

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However, water based poly has lower VOC’s (Volatile Organic Compounds) making it a “Greener” choice, but again, it’s a "catch 22", seeing how the life of your hardwood floor will be cut short, due to refinishing twice as often, ultimately leading to the cutting down of more trees in order to create new hardwood flooring