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They applied this gate integrating both transcriptional and translational switches (including ncAA 3iY, inducible promoter P tac or constitutive promoter P tyrRS drives RS, inducible promoter P T7 or constitutive promoter P Ipp drives tRNA, and gene encoding EGFP or ColE3e with zero to three amber codons inserted next to the start codon which is driven by P T7 promoter) to construct a gene expression platform capable of producing significant protein quantities upon induction conditions, while maintaining zero-leakage expression during repression conditions

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