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24 h later, cells expressing gH/gL, gH/gL/gp42, gp350 or mock-transfected cells were pelleted by centrifugation at 500 × g for 5 min and resuspended at 10 6 cells/ml in PBS containing 2% FBS and 1 mM EDTA (FACS buffer), then plated at 100,000 cells per well