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To determine the assay window for the FP assay, the TIN2-FAM probe was prepared as a 16-µM stock in aqueous buffer and titrated from 0 to 400 nM against either a constant concentration of TRF1 TRFH (0.5 µM in FP buffer) or FP buffer alone (25 mM HEPES-NaOH pH 7.5, 100 mM NaCl, 1 mM TCEP, 0.05% w/v CHAPS)