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To assess the LMW persulfides, 100 μL samples were precipitated with 1 mL of absolute MeOH at –40 °C for 2 h, centrifuged (16,000 × g at 0 °C), and obtained supernatants were evaporated and crude residues were resuspended and sonicated in 100 μL 50 mM HEPES buffer (pH 7.4)

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Gut 1994;35:696–700