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However, upon release in the hydrophilic cytoplasm, the fluorescence of the dye is quenched, allowing for the facile detection of the degradation of the nanoparticles

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Because of the non-identity of the different transitions monitored, the acid denaturation of BaGSTM does not appear to be a simple two-step transition, rather a multi-step process during which several intermediates coexist in equilibrium