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After further culturing for 24 h, each well was washed, 1 mg mL −1 MTT solution (100 µL) was added, and the cells were re-incubated for 4 h in the dark

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Although elevations in total cholesterol concentrations of 8% can negate the protection against coronary heart disease provided by a 5 mmHg reduction in blood pressure, data from long-term studies suggest diuretic-induced cholesterol changes are not clinically significant and do not contribute to coronary heart disease risk