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The eluants were reconstituted in water/acetonitrile/FA (solvent E, 10 µL, 98/2/0.1, v/v/v), and aliquots (5 µL) were injected onto a reverse phase nanobore HPLC column (AcuTech Scientific, C18, 1.8 μm particle size, 360 µm x 20 cm, 150 µm ID), equilibrated in solvent E and eluted (500 nL/minute) with an increasing concentration of solvent F (acetonitrile/water/FA, 98/2/0.1, v/v/v: min./% F