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Remove the liquid Antibody capture Add the antibody solution immediately Resuspend and mix for at least 15 minutes Remove the liquid Washing (repeat twice) Add 500 µl Binding Buffer containing 0.5 M NaCl Remove the liquid Target binding Add sample diluted in binding buffer Remove and collect the unbound fraction Washing Add 500 µl Binding Buffer containing 0.5 M NaCl Remove the liquid Elution Add 2 volumes of elution buffer For a minimum of two minutes, resuspend and incubate the beads Remove and collect the elution fraction The same steps and reagents can be followed with protein A/G magnetic beads