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Metabolome analysis (C-SCOPE) Metabolome analysis was conducted according to HMT’s C-SCOPE package, using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) for cation analysis and CE-tandem mass spectrometry (CE-MS/MS) for anion analysis, based on previously described methods 36,37

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Gnarl3-3xFLAG expression was induced by the addition of 1 mM IPTG one hour later, and cells were centrifuged at 8000 × g for 10 min after another hour

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