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These findings have general implications for the use of roGFP2-based redox probes: as sulfane sulfur species can oxidize roGFP2-Orp1 in an Orp1-independent manner, it is important to compare fused and unfused roGFP2 probes in side-by-side experiments to distinguish between Orp1-dependent and Orp1-independent roGFP2 oxidation events (as demonstrated in Fig

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