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All TMT labeled samples were combined in equal amounts in a new tube (200 µg) and were run on Mass spec after fractionation

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mTORC2 complex is composed by the rapamycin-insensitive companion (Rictor) (25), a component essential for both, complex formation, and their biological function, the mammalian stress-activated map kinase-interacting protein 1 (mSin1) an essential component required for complex formation and kinase activity (26), and by Protor 1 (Protein observed with Rictor 1), required to allow efficient regulation of mTORC2 targets (27)