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MM/PBSA (Molecular Mechanics/Poisson-Boltzmann Surface Area) calculates the free energy difference between the ligand and the protein-ligand complex in various states, which helps evaluate the binding affinity of a ligand to a protein

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While the persisters had an overall reduction in protein synthesis compared to parental cells, they translated certain pro-survival mRNAs, including chromatin remodelers and stress-responsive kinases, at increased efficiencies 36