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The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000

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The sulfur atom of the N-terminal cysteine of the CXXC motif (S1 in Figure 8), also known as the interchange cysteine, is solvent accessible, whereas its redox partner (S2) is typically buried within the protein