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For samples frozen on holey carbon grids, the Vitrobot was maintained at 4 °C with 100% humidity, using blotting times of 3.5 s to 4.5 s, blotting force of 0, and a waiting time of 10 s

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AUTHOR’S CONTRIBUTIONS All authors contributed greatly to the success of this work CONFLICT OF INTEREST No conflict of interest to declare in this manuscript ACKNOWLEDGEMENT This case was carried out without direct funding, and the authors would like to thank the Head and the entire staffs of the Department of Human Physiology, College of Medical Sciences, Ahmadu Bello University, Zaria, for their support and provision of necessary facilities